Comparative Evaluation of the Efficacy of Irrigation Delivery Systems on Enterococcus faecalis

Raksha Bhat, Preethesh Shetty, Mithra N. Hegde


Attaining a sterile root canal system is important since microorganisms can cause persistent inflammation in theperiradicular tissues. Objectives: This study sought to evaluate the efficacy of two irrigation delivery systems;the EndoVac and semiconductor diode laser irradiation with 5.25% sodium hypochlorite and 2% chlorhexidine. Methods: Fifty teeth were disinfected according to the Occupational Safety and Health Administration regulations.The teeth were then instr umented, followed by inoculation with bacterial strains of Enterococcus faecalis (ATCC29212). The teeth were randomly assigned to five groups, Group I: Control group; Group II: Teeth irrigated with5.25% NaOCl + Endovac; Group III: Teeth irrigated with 2% Chlorhexidine + Endovac; Group IV: Teeth irrigatedwith 5.25% NaOCl + laser irradiation; and Group V: Teeth irrigated with 2% Chlorhexidine laser irradiation. Allsamples were incubated on Muller–Hilton media plates for a period of 24 h. We determined the colony-formingunits and analyzed them statistically using Fisher’s exact test. Results: Laser irradiation completely disinfectedthe root canal system. The EndoVac system produced significant disinfection but was comparatively less effectivethan laser irradiation. Conclusion: Laser irradiation had a significantly greater bactericidal effect than the EndoVacsystem, used in conjunction with sodium hypochlorite and chlorhexidine.


Disinfection, Root canal system, Laser irradiation, Endovac, E.faecalis

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