Incidence of HPV Infection in Oral Squamous Cell Carcinoma and Its Association with the Presence of p53 & c-myc Mutation : A Case Control Study in Muwardi Hospital Surakarta

Adi Prayitno, Mandojo Rukmo, Ambar Mudigdo, Dalono Dalono, Elyana Asnar, Retno Puji Rahayu, Joko Agus Purwanto, Ni Nyoman Tri Puspaningsih, Kuntoro Kuntoro, Harjanto Harjanto, Suhartono Taat Putra

Abstract


Introduction: Annual incidence rates for oral and pharyngeal cancer are estimated at 25 cases per 100,000 in developing countries. Human papilloma virus (HPV) was implicated in pathogenesis of Cancer. The mutations of p53 and c-myc are found 50% in cancer. Objective: Aims of this research were to know the incidence of OSSC patient which realized HPV infection without p53 and c-myc gene mutation. Materials and Methods: Tissue biopsy frozen sections were taken from BOSC (Benign Oral Squamous Cell) and OSCC (Oral Squamous Cell Carcinoma) patients collected from Oral and Dental Departement of dr Muwardi Distric Hospital in Surakarta from January 2007 to January 2008. To amplify L1-HPV gene for fixed the HPV stressor. To amplify p53 and c-myc genes, continued with SSCP (Single Strand Conformational Polymorphisme) analysis and followed with measurement using densitometer, to see mutation existence. The collected data were analyzed with Chi Square. Results: BOSC patient identified 23% with HPV infections and OSCC patient identified 73% with HPV infections. Hundred percent BOSC patient with HPV infection without mutation in p53 gene and c-myc gene, 81% OSCC patient with HPV infection without mutation in p53 gene and 91% OSCC patient with HPV infection without mutation in c-myc gene. Chi  square analysis showed significant difference between BOSC and OSCC patients with HPV infection without mutation in p53 and c-myc gene. Conclusion: HPV is a factor for pathogenesis of OSCC.

DOI: 10.14693/jdi.v17i2.44


Keywords


developing countries; HPV; pathogenesis; OSCC

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