PCR-SSCP Analysis and DNA Sequencing as an Important Method for Detecting Mutation of INGI gene in Head and Neck Squamous Cell Carcinomas

Elza Ibrahim Auerkari, Mehmet Gunduz, Mamoru Ouchida

Abstract


Tumor specific mutation of the candidate tumor suppressor gene INGI ws demonstrated using PCR-SSCP and DNA sequencing analysis. The mutant sample showed an amino acid change from aspangine (AAC) to serine (AGC) at codon 214 in the INGI gene from the DNA of head and neck cancer cells. The result proved that PCS (polymerase chain reaction) - SSCP (single-stranded conformation polymorphism) analysis can effectively detect both wild and mutant types of the gene from tissue samples. Moreover, the nucelotide change can be determined by sequencing the mutant product. The purpose of this report is to illustrate the importance of SSCP and DNA sequencing in identifying nuclotide substitution in human head and neck carcinomas.

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